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dc.contributor.authorIsbell, Sydney
dc.contributor.authorMendonsa, Adrian
dc.contributor.authorCash, Kevin J.
dc.date2023-04
dc.date.accessioned2023-05-24T20:56:22Z
dc.date.available2023-05-24T20:56:22Z
dc.identifier.urihttps://hdl.handle.net/11124/176976
dc.identifier.urihttps://doi.org/10.25676/11124/176976
dc.description.abstractDiagnostic tests to determine analyte concentration can be repetitive and require extensive training for proper analysis. To address these limitations, we developed two ratiometric nanosensors (calcium (Ca2+) and oxygen (O2)) which could be implemented in-vivo to give insight into biological functions such as nerve signaling and cellular respiration. The Ca2+ nanosensors’ optical properties (fluorescence and absorbance) vary to reflect the surrounding Ca2+ concentration. These sensors are selective to Ca2+ over other biologically relevant cations (Mg2+, Na+, K+) and show a sensitivity to Ca2+ at concentrations as low as 100 µM. The O2 nanosensor is composed of two dyes encapsulated in a hydrophobic PVC matrix. The O2 sensitive dye, platinum octaethylporphyrin (PtOEP), shows a decrease in luminescence with increasing oxygen concentrations. Whereas, the reference dye, DiA, has no O2 sensitivity. These O2 sensors are reversible and have a detection range that spans from anoxic (0% O2) to atmospheric conditions (21% O2). While the Ca2+ and O2 sensors showed functionality in in-vitro studies, testing these sensors in-vivo will determine their effectiveness as a long-term diagnostic aid.
dc.format.mediumposters
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado School of Mines. Arthur Lakes Library
dc.relation.ispartof2023 Spring Undergraduate Research Symposium
dc.rightsCopyright of the original work is retained by the author.
dc.titleDevelopment of calcium and oxygen nanosensors for in-vivo diagnostics
dc.typeText
dc.typeStillImage


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