• Login
    View Item 
    •   Home
    • Theses & Dissertations
    • 2016 - Mines Theses & Dissertations
    • View Item
    •   Home
    • Theses & Dissertations
    • 2016 - Mines Theses & Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Mines RepositoryCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    Login

    Mines Links

    Arthur Lakes LibraryColorado School of Mines

    Statistics

    Display Statistics

    Microbial insights into enhanced trace organic biodegradation potential in water treatment

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Homme_mines_0052E_10983.pdf
    Size:
    3.129Mb
    Format:
    PDF
    Download
    Author
    Homme, Carissa L.
    Advisor
    Sharp, Jonathan O.
    Date issued
    2016
    Keywords
    biofiltration
    biostimulation
    nitrosamines
    propane monooxygenase enzyme
    trace organic chemicals
    
    Metadata
    Show full item record
    URI
    https://hdl.handle.net/11124/170098
    Abstract
    The widespread detection of anthropogenic trace organic chemicals (TOrCs) in environmental and drinking water supplies presents a critical challenge to current water reuse technology. While many of these compounds are susceptible to biodegradation, microbiological responses to relevant biostimulation methods remain poorly understood. The research presented here explores the viability of propane biostimulation during water treatment by an integrative approach coupling a well described pure bacterial strain to microbial community characterization and enzyme queries in more complex biofiltration systems relevant to managed and passive water treatment scenarios. Induction of the propane monooxygenase enzyme (PrMO) and its large subunit prmA in a common soil bacterium led to the biotransformation of multiple N-nitrosamines, a class of carcinogenic and mutagenic TOrCs. Kinetic analyses revealed hierarchical biodegradation rates that decreased with increased N-nitrosamine molecular weight and cyclic configuration that may also hold true in more complex systems. Flow-through columns employing municipally derived biologically active carbon with augmented propane as an operational variable exhibited ecological differentiation compared to control columns. However, this selective bias was limited to a relatively modest depth (< 15 cm) suggesting limited establishment of reactive zones in flow-through systems. Examination of microbial genera enriched in response to propane introduction identified groups potentially beneficial to a wide array of bioremediation applications as well as greater PrMO density. Finally, propane biostimulation and organic carbon limitation in flow-through sediment columns also impacted ecological selection and increased prmA gene abundance in column infiltration zones. Interestingly both propane biostimulation and carbon limitation similarly enhanced the attenuation of caffeine, carbamazapine, and sulfamethoxazole when contrasted with columns amended with pyruvate. This suggests that while propane biostimulation enhances TOrC attenuation and imparts ecological bias, similar functional gains that are potentially easier to engineer and finance can be achieved in a carbon-limited system.
    Rights
    Copyright of the original work is retained by the author.
    Collections
    2016 - Mines Theses & Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.