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Self-characterization of spatial frequency modulation imaging for multiphoton microscopy
Davidson, Jaden T.
Davidson, Jaden T.
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2021
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This thesis presents the results of introducing spectral differentiation into an optical method called SPIFI (spatial frequency modulated imaging). SPIFI is a method defined by its use of a special modulation mask, which varies in time and creates a correlation between spatial and temporal variables. In experimental tests, it was recorded that adding spectral dependence to SPIFI creates functionality similar to that of SEATADPOLE.SEATADPOLE is another imaging method, based on spectral interferometry, that is most often used to measure dispersion within signals. Such dispersion measurements are encoded in turn as correlation between the frequency of incoming light and spatial variables. The method has been investigated and verified to bring various benefits to the field of optical imaging.
Our primary goal was to combine the two methods in order to confirm that a SEATADPOLE trace, and the related spatial and temporal measurements, can be extracted from SPIFI. This is important because it allows for a rigorous level of self-characterization of the focal intensity within the system. In order to check that the hypothesis is theoretically viable, preliminary tests were done through the Zemax Optic Studio interface. This highlights many valuable parameters within the system, including aberration, the specific values of dispersion, and the light beam cross section through the system as explained by theory. The experiment was then repeated in a laboratory setup. Images taken in this system correspond to the most important theoretical cross-sections and measurements, including the SEATADPOLE trace. Overall, the main hypothesis is clearly confirmed, with a baseline SEATADPOLE trace identified and verified against theoretical tests, based on a system with no sample; the results that do not agree perfectly also can serve as a source of new information upon which the methods can be refined and further developed.
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