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Video rate spatial frequency modulation imaging
Kingsolver, Ian Mars
Kingsolver, Ian Mars
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2021
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This thesis discusses the first Spatial Frequency Modulation Imaging (SPIFI) system capable of collecting data at video rate speeds. In the brief history of SPIFI, systems have been limited in imaging dynamic samples due to line imaging rates in the range of 20 to 30 hertz. The creation of a Video Rate SPIFI (V-SPIFI) system, utilizing a stationary reflective rectangular modulation plane and an air-bearing polygonal scan mirror, allows for dynamic sample imaging with concurrent data availability. The resulting V-SPIFI system is capable of capturing line images over 240 times faster than previous SPIFI systems. By implementing a prototype electronic optical control subsystem, the V-SPIFI system is also capable of automatically scanning a sample and rendering 25 first and second harmonic order images in approximately five seconds. To provide context, a brief history of modern microscopy techniques and limitations is included. Next, using the theory of SPIFI imaging, the modeling and construction of the six interconnected optical and electronic subsystems required for the V-SPIFI system are detailed. Resulting first and second order harmonic images are presented, including pixel size and estimated first order image resolution. A visual verification of image accuracy is also provided. Finally, concepts for future V-SPIFI systems are discussed, as well as possible applications of the V-SPIFI system.
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