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Fluorescent point tracking for optical encoding in near-field ptychography

Masztalerz, Veronica
Mubeen, Nabeeha
Selman, Beau
Adams, Daniel
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2024-04
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Abstract
Ptychography is a process that utilizes scattered light patterns to image an object. To perform ptychography, an optical stage is employed to shift an object relative to a laser, often in hundreds of discrete positions. Keeping track of this motion involves a system of encoding the stage's position at each shift. This research focuses on optical encoding by tracking the motion of a fluorescent point adjacent to the stage. By imaging the fluorescent point at each position along with computing a cross-correlation with prior positions, information about the fluorescent point's relative position is calculated. Thus, the difference between highest-intensity positions at each stage motion represents the length traveled by the stage, effectively encoding its movement.
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