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Optimizing residence time for recalcified blood in a microfluidic mixer
Huber, Rebecca E. Sorrells, Matthew Neeves, Keith B.
Huber, Rebecca E.
Sorrells, Matthew
Neeves, Keith B.
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2017-07
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Abstract
Many research laboratories are currently using microfluidic devices to model blood vessels to gain a better understanding of blood disorders and how to treat them. By using a continuous recalcification method, experimentation time can be elongated, and more complex systems can be studied. In order to use this method effectively, it should be known how long the blood and the recalcification buffer should be mixing for minimal platelet activation, but still hold the ability to activate given a chemical stimulus. By using varying lengths of effluent tubing, different residence times were studied to give an improved understanding of how the blood reacts upon recalcification. By labeling the effluent blood samples with antibodies, flow cytometry can reveal the activation state of platelets. The results of this experiment suggest no significant difference in activation between residence times of one and ten minutes.
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